狭义的转录组默认只包含mRNA,但是广义的转录组指的是细胞或组织中所有转录产物的集合,其中包含mRNA和非编码RNA(non-coding RNA, ncRNA)。非编码RNA目前的研究多集中在具有调控功能的small RNA(以miRNA为代表),长链非编码RNA(long non-coding RNA, lncRNA)和环状RNA(circular RNA, circRNA)上,而这几类ncRNA的调控对象都和mRNA有关。因此,全转录组即包含了ncRNAs和mRNA。全转录组测序即对以上四种ncRNA进行测序,并分析这四者之间复杂的相互作用关系。

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